Practical: Environmental Biotechnology and Wastewater Analysis

Paper Code: 
24DENV602A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to:

  1. Develop students' skills in the design, implementation, and interpretation of experiments related to environmental biotechnology applications
  2. Develop students' skills for physicochemical quality assessment of wastewater for planning monitoring programmes for pollution prevention

Course Outcomes (COs):

Course

Course Outcomes

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

24DENV 602A

Practical: Environmental Biotechnology and Wastewater Analysis

CO72: Develop skills in laboratory

techniques for the isolation and

characterization of DNA involved

in environmental biotechnology

processes

CO73: Apply techniques for the

extraction     and     analysis     of environmental pollutants using

chromatographic methods

CO74: Demonstrate and quantify

the physiological parameters for

waste    water    treatment    and

discharge

CO75: Communicate scientific information related to environmental biotechnology in oral and written formats, including the preparation of laboratory reports

CO76: Contribute effectively in course-specific interaction

Approach              in teaching:

Conduction              of practical       exercises, Preparation of sample dilutions, demonstration

Learning activities for the students: Simulation, Presentation, Self- learning videos

Preparation of practical records, Viva Voce, Class Test, Semester End Examination

 

CONTENTS

  • Isolation of Lambda DNA using Agarose gel electrophoresis
  • Restriction digestion of DNA
  • Separation of Amino acids mixture using Thin Layer Chromatography (TLC)
  • Separation of dyes mixture using Paper chromatography
  • Estimation of Chlorine demand in the wastewater sample
  • Estimation of Dissolved oxygen in the wastewater sample
  • Estimation of Biochemical Oxygen Demand in the wastewater sample
  • Estimation of Chemical Oxygen Demand in the wastewater sample
  • Estimation of Total suspended solids in the wastewater sample
  • Quantitative estimation of nitrates in the wastewater sample
  • Quantitative estimation of phosphates in the wastewater sample

Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above.

 

Academic Year: 
2024-2025
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