PRACTICAL
Objective:
- Preparation of Nutrient Agar and Nutrient Broth
- Isolation of pure culture using Streak Plate Technique
- Isolation of microorganisms from soil sample.
- Isolation of microorganisms from water sample
- Isolation of microorganisms from food sample
- Examination of sanitary quality of milk by coliform analysis and methylene blue reductase test
- To study the gram staining of bacteria culture
- Thin layer chromatography
- Paper chromatography
- MPN (Most Probable Number)-for polluted and non polluted water samples
- Acquaintance with the following for spot identification
- Autoclave
- Laminar flow hood
- Incubator
- Hot plate
- pH meter
- Centrifuge
- Magnetic Stirrer
- Inoculating loop
- Oven
- Bunsen burner
- Qubec Colony counter