Coming soon
Practical
Course objectives
This course will enable the students to -
1. Integrate theoretical and practical knowledge of isolation of microbes from different habitats
2. Make the students aware about natural disasters and their management
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessme nt Strategies |
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|---|---|---|---|---|
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Paper Code |
Paper Title |
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ENV 325 |
Practical |
Upon completion of the practical course, student will: CO97: Develop understanding regarding working principle and procedures for instruments used in microbiology and biotechnology exercises CO98: Demonstrate techniques involved in isolation of microorganisms from different habitats CO99: Devise techniques for quantification of microbes from various water sources CO100: Categorize natural disasters and describe their management process CO101: Appraise the natural and anthropogenic disasters and analyze their management process through report preparation and presentation. CO102: Examine the disasters of the past and critically analyze them through case studies and map plotting |
Approach in teaching: Demonstration, Group discussions, Conducting experiments, field trips Learning activities for the students: Brainstorming, preparation of maps, reports, individual and group projects, surveys |
Spotting, Quiz Power Point Presentation, Map Activity, Viva Voce, Class test and Semester end examination |
CONTENTS MICROBIOLOGICAL ANALYSIS
· Acquaintance with the following for spot identification:
Autoclave, Incubator, Hot plate, Water bath, Stirrer, Inoculating loop, Oven, Centrifuge, Bunsen burner, Laminar flow hood, Microscope
· Determination of coliforms and fecal streptococci in water and wastewater by Multiple fermentation technique
· Detection of coliforms and fecal coliforms in water by the membrane filtration method
· Differentiation of members of family Enterobacteriaceae using IMViC tests
· Heterotrophic plate count
· Isolation and enumeration of microorganisms from soil by serial dilution agar plating method
BIOTECHNOLOGY
· Thin layer chromatography
· Gel Electrophoresis
EXERCISE ON DISASTER MANAGEMENT
· The students will prepare a report and a power point presentation on specific disaster management topics of interest (eg. Earthquake, Floods, Drought, Landslide, epidemic, pandemic, anthropogenic disasters, etc.) followed by peer feedback and discussions on presentation content and delivery.
· Research and compilation of a list of essential items needed in an emergency preparedness kit followed by discussions and review of the contents of the preparedness kit
· Case study on any recent disaster including analysis of the disaster response and recovery efforts, including the successes and failures
· Earthquake drill: Simulate an earthquake scenario and practice the appropriate safety procedures
MAP FILE FOR DISASTER MANAGEMENT
· Flood prone areas in India
· Major floods in India
· Landslide prone areas in India
· Major landslides in India
· Cyclone prone areas in India
· Major cyclones in India
· Drought prone areas in India
· Seismic zones of India
· Major earthquakes in India
· Major anthropogenic disasters in India
· Major anthropogenic disasters in world
· Major riots in India
· Major terrorists’ attacks in India
· Major mining accidents in India
· Major nuclear disasters in world
· Major active volcanoes in world
LABORATORY READINGS:
· Adams,Danny Spencer,LabMath:A Handbook of Measurements, Calculations and Other Quantitative Skills for Use at the Bench
· Laboratory Manual in General Microbiology-Kannan, N
