Environmental Microbiology and Biotechnology

Paper Code: 
ENV 321
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives

This course will enable the students to –

  1. Impart knowledge about microbial diversity in various environments
  2. Understand the role of microorganism in degradation of xenobiotics, mineral recovery, waste management, biotechnology

Course Outcomes (COs):

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ENV 321

Environmental Microbiology and Biotechnology

Upon completion of this

course, student will:

CO73: Appraise the diversity

of microorganisms and learn

about        the                 abundance

distribution and significance of

microorganism        in the

environment

CO74: Categorize the diversity adaptations         and

biotechnological applications of microbes of extreme environment

CO75: Explain microbiological

solutions        to        address

environmental issues including

pollution and degradation of

xenobiotics

CO76: Illustrate the role of

microbes         in nutrient

transformation mineral

resource      mining, waste

management         and

biotechnology

CO77: Explain the importance

of microbial diversity and molecular approaches in dealing with environmental systems, processes and biotechnology

CO78: Devise biotechnological solutions to address environmental issues including pollution, land reclamation and

waste management

Approach in

teaching:

Interactive

lectures, Slide

Presentation,

Demonstrations,

Informative videos

Learning activities for the students:

Group discussions, Problem solving, case studies, simulation

Quiz, Assignments, Power point presentations, open book test, tutorials class test, semester end examination

 

10.00
Unit I: 
Introduction to Environmental Microbiology
  • Phases of bacterial growth (Lag phase, Exponential phase and Stationary and Death phase)
  • Types of microorganisms on the basis of Nutrition
  • Factors affecting growth of microorganisms
  • Types of microorganisms on the basis of osmotic concentration, pH, temperature and oxygen concentration and pressure.
12.00
Unit II: 
Microbial Diversity of Air
  • Microbial communities in air and their sources
  • Physical/Microbial habitats in air
  • Factors affecting microbial survival in air
  • Aero-microbiological Pathway
  • Launching of bioaerosols
  • Transport and Dispersion
  • Deposition
  • ·        Mechanisms to control Bioaerosols
  • Ventilation
  • Filtration
  • Biocidal Control and Isolation
14.00
Unit III: 
Microbial Degradation of Xenobiotic Compounds
  • Factors affecting degradation of xenobiotics
  • Aerobic degradation of xenobiotics
  • Degradation of Aliphatic compounds
  • Degradation of Aromatic compounds
  • Anaerobic degradation of xenobiotics
  • Degradation of Aliphatic compounds
  • Degradation of Aromatic compounds
  •    Microbial degradation of Halogenated Compounds
12.00
Unit IV: 
Environmental Biotechnology
  • Concept of Recombinant technology
  • Molecular tools of genetic engineering
  • Vectors-Plasmids (pBR322), Bacteriophages {lambda (λ} Cosmids, BAC and YAC
  • Methods of gene transfer – Conjugation, Transformation, Transduction.
  • Basic Concept of Metagenomics
12.00
Unit V: 
Applications of Environmental Microbiology & Biotechnology
  • Bioremediation-In-situ and Ex-situ
  • Phytoremediation
  • Bioleaching (methods and microorganisms used
  • Integrated Pest Management (Concept of IPM, Physical, Cultural, Biological and Chemical methods of pest management).
  • Biosensors-Types and application
Essential Readings: 

ESSENTIAL READINGS

  • Maier, R.M., I. L. (2009). Environmental Microbiology (2nd ed.). USA: Academic Press (Elsevier).
  • Michael J. Pelczar, E. C. (1993). Microbiology (5th ed.). New Delhi: Tata McGraw-Hill Publishing Company Limited.
  • Mohapatra, P. K. (2008). Textbook of Environmental Environmental Microbiology. New Delhi: I. K. International Publishing House.
  • Mohapatra, P. K. (2010). Textbook of Environmental Biotechnology. New Delhi: I.K. International Pvt. Ltd.

SUGGESTED READINGS

  • Atlas, R.M., R. B. (2005). Microbial Ecology: Fundamental and Applications (4th ed.). New Delhi: Pearson Education Pvt. Ltd.
  • Barton, L., D. E. (2011). Microbial Ecology. New Jersey: John Wiley & Sons, Inc., Publication..
  • Evans, G. M., & Furlong, J. C. (2003). Environmental Biotechnology: Theory and Application. John Wiley & Sons Ltd.
  • Thakur, I. S. (2011). Environmental Biotechnology: Basic Concepts and Applications. New Delhi: I.K. International Pvt. Ltd.
  • Wainwright, M. (1999). An Introduction To Environmental Biotechnology. New York: Springer Science+Business Media, Llc.

e. RESOURCES

Academic Year: 
2023-2024
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