- Bacteria (Lactic acid production, Vinegar production, Amino acid
production),
-Yeast (Alcohol fermentation, Bakers yeast and food yeast),
-Moulds (Penicillin production, Citric acid production, Enzyme production).
-Intracellular accumulation
- Cell wall associated metal interaction
-Metal- siderophore interactions
-Extracellular mobilization/immobilization of metals by bacterial metabolites
-Extracellular polymer-metal interactions
- Transformation and volatilization of metals
-Molecular tools of genetic engineering
-Host cell-Prokaryotic and Eukaryotic
-Vectors-Plasmids(pBR322),Bacteriophages(λ)Cosmids, BAC amd YAC
-Methods of gene transfer – Conjugation,Transformation,Transduction.