Coming soon
PRACTICAL
This course will enable the students to -
- Integrate theoretical and practical knowledge of isolation of microbes from different habitats
- Make the students aware about natural disasters and their management
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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|---|---|---|---|---|
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Paper Code |
Paper Title |
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ENV 325 |
Practical |
Upon completion of the practical course, student will: CO97: Develop understanding regarding working principle and procedures for instruments used in microbiology and biotechnology exercises CO98: Demonstrate techniques involved in isolation of microorganisms from different habitats CO99: Devise techniques for quantification of microbes from various water sources CO100: Categorize natural disasters and describe their management process CO101: Appraise the natural and anthropogenic disasters and analyze their management process through report preparation and presentation. CO102: Examine the disasters of the past and critically analyze them through case studies and map plotting |
Approach in teaching: Demonstration, Group discussions, Conducting experiments, field trips Learning activities for the students: Brainstorming, preparation of maps, reports, individual and group projects, surveys |
Spotting, Quiz Power Point Presentation, Map Activity, Viva Voce, Class test and Semester end examination |
CONTENTS
MICROBIOLOGICAL ANALYSIS
- Acquaintance with the following for spot identification: Autoclave, Incubator, Hot plate, Water bath, Stirrer, Inoculating loop, Oven, Centrifuge, Bunsen burner, Laminar flow hood, Microscope
- Determination of coliforms and fecal streptococci in water and wastewater by Multiple fermentation technique
- Detection of coliforms and fecal coliforms in water by the membrane filtration method
- Differentiation of members of family Enterobacteriaceae using IMViC tests
- Heterotrophic plate count
- Isolation and enumeration of microorganisms from soil by serial dilution agar plating method
BIOTECHNOLOGY
- Thin layer chromatography
- Gel Electrophoresis
EXERCISE ON DISASTER MANAGEMENT
- Meteorological: Hailstorm, Snow storm, Dust storm, Wind storm, Fog, Lightening, Heavy rain
- Geological: Earthquake, Landslide, Mudflow, Tsunami, Volcano;
- Hydrological: Drought, Flood
- Biological: Epidemics, Pest
- Infrastructure: Transport -Aviation, Railway, Road, Shipping, Energy -Mining, Nuclear
- Industrial: Chemical, Gas Leak
- Others: Fire, Riot, Terrorism, War
- The students will prepare a report and a power point presentation on the topics allocated to them.
- They will also prepare a map file based on disaster management exercises.
LABORATORY READINGS:
- Adams,DanySpencer,LabMath:A Handbook of Measurements, Calculations and Other Quantitative Skills for Use at the Bench
- Laboratory Manual in General Microbiology-Kannan, N
Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above
