PRACTICAL

Paper Code: 
ENV 325
Credits: 
8
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to -

  1. Integrate theoretical and practical knowledge of isolation of microbes from different habitats
  2. Make the students aware about natural disasters and their management

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ENV 325

Practical

Upon completion of the practical course, student will:

CO97:  Develop understanding regarding working principle and procedures for instruments used in microbiology and biotechnology exercises

CO98:  Demonstrate techniques involved in isolation of microorganisms from different habitats

CO99:  Devise techniques for quantification of microbes from various water sources

CO100: Categorize natural disasters and describe their management process

CO101:  Appraise the natural and anthropogenic disasters and analyze their management process through report preparation and presentation.

CO102: Examine the disasters of the past and critically analyze them through case studies and map plotting

Approach in teaching:

Demonstration, Group discussions, Conducting experiments, field trips

Learning activities for the students:

Brainstorming, preparation of maps, reports, individual and group projects, surveys

Spotting, Quiz

Power Point Presentation, Map Activity, Viva Voce, Class test and Semester end examination

CONTENTS

MICROBIOLOGICAL ANALYSIS

  • Acquaintance with the following for spot identification: Autoclave, Incubator, Hot plate, Water bath, Stirrer, Inoculating loop, Oven, Centrifuge, Bunsen burner, Laminar flow hood, Microscope
  • Determination of coliforms and fecal streptococci in water and wastewater by Multiple fermentation technique  
  • Detection of coliforms and fecal coliforms in water by the membrane filtration method
  • Differentiation of members of family Enterobacteriaceae using IMViC tests
  • Heterotrophic plate count
  • Isolation and enumeration of microorganisms from soil by serial dilution agar plating method

 BIOTECHNOLOGY

  • Thin layer chromatography
  • Gel Electrophoresis

EXERCISE ON DISASTER MANAGEMENT

  1. Meteorological: Hailstorm, Snow storm, Dust storm, Wind storm, Fog, Lightening, Heavy rain
  2. Geological: Earthquake, Landslide, Mudflow, Tsunami, Volcano;
  3. Hydrological: Drought, Flood
  4. Biological: Epidemics, Pest
  5. Infrastructure: Transport -Aviation, Railway, Road, Shipping, Energy -Mining, Nuclear
  6. Industrial: Chemical, Gas Leak
  7. Others: Fire, Riot, Terrorism, War
  • The students will prepare a report and a power point presentation on the topics allocated to them.
  • They will also prepare a map file based on disaster management exercises.

LABORATORY READINGS:

  • Adams,DanySpencer,LabMath:A Handbook of Measurements, Calculations and Other Quantitative Skills for Use at the Bench
  • Laboratory Manual in General Microbiology-Kannan, N

Note:  Any other practical exercise (related to theory) may be conducted besides the ones mentioned above

 

Academic Year: 
2021-2022
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