PRACTICAL

Paper Code: 
ENV 325
Credits: 
8
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 

Course objectives

This course will enable the students to -

  1. Integrate theoretical and practical knowledge of isolation of microbes from different habitats
  2. Become aware about natural disasters and their management

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ENV 325

Practical

Upon completion of the practical course, student will be able to:

CO65:  Develop understanding regarding working principle and procedures for instruments used in microbiology and biotechnology exercises

CO66:  Demonstrate techniques involved in isolation of microorganisms from different habitats

CO67:  Develop a very good understanding of quantification of microbes from various water sources

CO68:  Identify natural disasters and describe their management process

Approach in teaching:

Demonstration, Group discussions, Conducting experiments, field trips

Learning activities for the students:

Brainstorming, preparation of maps, reports, individual and group projects, surveys

Spotting, Quiz

Power Point Presentation, Map Activity, Viva Voce, Class test and Semester end examination

 

 

MICROBIOLOGICAL ANALYSIS

  • Acquaintance with the following for spot identification:Autoclave, Incubator, Hot plate, Water bath, Stirrer, Inoculating loop, Oven, Centrifuge, Bunsen burner, Laminar flow hood, Microscope
  • Determination of coliforms and fecal streptococci in water and wastewater by Multiple fermentation technique
  • Detection of coliforms and fecal coliforms in water by the membrane filtration method
  • Differentiation of members of family Enterobacteriaceae using IMViC tests
  • Heterotrophic plate count
  • Isolation and enumeration of microorganisms from soil by serial dilution agar plating method

 BIOTECHNOLOGY

  • Thin layer chromatography
  • Gel Electrophoresis

EXERCISE ON DISASTER MANAGEMENT

1.Meteorological :Hailstorm, Snow storm, Dust storm, Wind storm, Fog, Lightening, Heavy rain

2.Geological : Earthquake, Landslide, Mudflow, Tsunami, Volcano;

3.Hydrological :Drought ,Flood

4. Biological: Epidemics, Pest

5. Infrastructure :Transport -Aviation ,Railway, Road, Shipping, Energy -Mining ,Nuclear

6.Industrial :Chemical ,Gas Leak

7. Others :Fire, Riot, Terrorism, War

The students will prepare a report and a power point presentation on the topics allocated to them. They will also prepare a map file based on disaster management exercises.

Note:  Any other practical exercise (related to theory) may be conducted besides the ones mentioned above

 

 

Essential Readings: 
  • Adams,Dany Spencer,Lab Math:A Handbook of Measurements, Calculations and Other Quantitative Skills for Use at the Bench
  • Laboratory Manual in General Microbiology Kannan, N

 

Academic Year: 
2020-2021
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