Practical

Paper Code: 
DENV 602A
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to:

  1. Develop students' skills in the design, implementation, and interpretation of experiments related to environmental biotechnology applications
  2. Develop students' skills for physicochemical quality assessment of wastewater for planning monitoring programmes for pollution prevention

Course Outcomes (COs):

Course

Course outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

DENV 602 A

Practical

Upon             completion          of             this

practical                  course,                  students

will:

CO53: Develop skills in laboratory

techniques for the isolation and

characterization of DNA involved

in environmental biotechnology

processes

CO54: Apply techniques for the

extraction    and                   analysis               of environmental pollutants using

chromatographic methods

CO55: Demonstrate and quantify

the physiological parameters for

waste   water            treatment            and

discharge

CO56: Communicate scientific information related to environmental biotechnology in oral and written formats, including the preparation of

laboratory reports

Approach             in teaching: Conduction             of practical                   exercises, Preparation of sample dilutions, demonstration

Learning activities for the students: Simulation, Presentation,                          Self- learning videos

Preparation of practical records, Viva Voce, Class Test, Semester End Examination

CONTENTS

  • Isolation of Lamba DNA using Agarose gel electrophoresis
  • Restriction digestion of DNA
  • Separation of Amino acids mixture using Thin layer chromatography (TLC)
  • Separation of dyes mixture using Paper chromatography
  • Estimation of Chlorine demand in the wastewater sample
  • Estimation of Dissolved oxygen in the wastewater sample
  • Estimation of Biochemical Oxygen Demand in the wastewater sample
  • Estimation of Chemical Oxygen Demand in the wastewater sample
  • Estimation of Total suspended solids in the wastewater sample
  • Quantitative estimation of nitrates in the wastewater sample
  • Quantitative estimation of phosphates in the wastewater sample
  • Estimation of Total Kjehldahl Nitrogen

Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above.

 

Academic Year: 
2023-2024
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