ENVIRONMENTAL MICROBIOLOGY AND BIOTECHNOLOGY

Paper Code: 
ENV 321
Credits: 
5
Contact Hours: 
75.00
Max. Marks: 
100.00
Objective: 

Course objectives

This course will enable the students to –

  1. Gain knowledge about microbial diversity in various environments
  2. Understand the role of microorganism in degradation of xenobiotics, mineral recovery, waste management, biotechnology

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ENV 321

 

Environmental Microbiology and Biotechnology

 

Upon completion of this course, student will be able to:

CO49: Appraise the diversity of microorganisms and learn about the abundance distribution and significance of microorganism in the environment

CO50: Describe the diversity adaptations and biotechnological applications of microbes of extreme environment

CO51: Explain microbiological solutions to address environmental issues including pollution, degradation of xenobiotics

CO52: Illustrate the role of microbes in nutrient transformation mineral resource mining, waste management and biotechnology

Approach in teaching:

Interactive lectures, Slide Presentation, Demonstrations, Informative videos

Learning activities for the students:

Group discussions, Problem solving, case studies, simulation

 

Quiz, Assignments, Power point presentations, open book test, tutorials class test, semester end examination

 

15.00
Unit I: 
Introduction to Environmental Microbiology
  • Scope and application of Environmental Microbiology
  • Phases of bacterial growth (Lag phase ,Exponential phase and Stationary and Death phase )
  • Types of microorganisms on the basis of Nutrition
  • Factors affecting growth of microorganisms & Types of microorganisms on the basis of osmotic concentration, pH, temperature and oxygen concentration and pressure
15.00
Unit II: 
Microbial Diversity of Air Microbial Diversity of Air
  • Microbial communities in air and their sources
  • Physical/Microbial habitats in air
  • Factors affecting microbial survival in air
  • Aeromicrobiological Pathyway
    • Launching of bioaerosols
    • Transport and Dispersion
    • Deposition
  • Mechanisms to control Bioaerosols
    • Ventilation
    • Filtration
    • Biocidal Control
    • Isolation

 

15.00
Unit III: 
Microbial Degradation of Xenobiotic Compounds
  • Factors affecting degradation of xenobiotics
  • Aerobic degradation of xenobiotics
    • Degradation of Aliphatic compounds
    • Degradation of Aromatic compounds
  • Anaerobic degradation of xenobiotics
    • Degradation of Aliphatic compounds
    • Degradation of Aromatic compounds
  • Microbial degradation of Halogenated Compounds      

 

15.00
Unit IV: 
Environmental Biotechnology
  • Concept of Recombinant technology
  • Molecular tools of genetic engineering
  • Vectors-Plasmids(pBR322),Bacteriophages(λ)Cosmids, BAC amd YAC
  • Methods of gene transfer – Conjugation,Transformation,Transduction.
  • Bioremediation-insitu and exsitu
  • Basic Concept of Metagenomics
15.00
Unit V: 
Biotechnology and Resource Conservation
  • Production of biogas- Biomethanation (Microbiology and biochemistry, Factors affecting biogas production, Design of digesters)
  • Composting (stages, types, and factors affecting the process)
  • Vermicomposting
  • Bioleaching (methods and microorganisms used)
  • Integrated Pest Management (Concept of IPM, Physical, Cultural, Biological and Chemical methods of pest management).
  • Biosensors-Types and applications

 

Essential Readings: 
  • ·         Atlas, R.M., R. B. (2005). Microbial Ecology: Fundamental and Applications (4th ed.). New Delhi: Pearson Education Pvt. Ltd.
  • ·         Barton, L., D. E. (2011). Microbial Ecology. New Jersey: John Wiley & Sons, Inc., Publication.
  • ·         Chatterji, A. (2011). Introduction to Environmnetal Biotechnology (3rd ed.). New Delhi: PHI Learning Pvt. Ltd.
  • ·         Evans, G. M., & Furlong, J. C. (2003). Environmental Biotechnology: Theory and Application. John Wiley & Sons Ltd.
  • ·         Jjemba, P. K. (2004). Environmental microbiology: principles and applications. Science Publishers.
  • ·         Maier, R.M., I. L. (2009). Environmental Microbiology (2nd ed.). USA: Academic Press (Elsevier).
  • ·         Michael J. Pelczar, E. C. (1993). Microbiology (5th ed.). New Delhi: Tata McGraw-Hill Publishing Company Limited.
  • ·         Mohapatra, P. K. (2008). Textbook of Environmental Environmental Microbiology. New Delhi: I. K. International Publishing House.
  • ·         Mohapatra, P. K. (2010). Textbook of Environmental Biotechnology. New Delhi: I.K. International Pvt. Ltd.
  • ·         Prescott, L. M. (2002). Microbiology (5th ed.). The McGraw−Hill Companies.
  • ·         Stanier, R. (1987). General Microbiology (5th ed.). Palgrave Macmillan .
  • ·         Wainwright, M. (1999). An Introduction To Environmental Biotechnology. New York: Springer Science+Business Media, Llc.

 

Academic Year: 
2019-2020
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