Research Methodology (Compulsory paper) LSC 141

Paper Code: 
LSC 141
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00
Unit I: 
Basics of Research

 

·         Research: Meaning, Methodology, research processes (basic steps), criteria of good research.

·         Types of research:  Fundamental or basic, applied, Historical, descriptive, analytical, qualitative, quantitative, experimental, conceptual, case study.

·         Research design: Meaning, concepts, needs, and designs for different types of research.

·         Research problem and developing research proposal: Selection of research area and topic, statement of the research problem, scope, and steps involved in defining the problem.

Literature search: reviewing related literature, referencing, abstracting, computer search, bibliography

12.00
Unit II: 
Basics of Statistics

 

  •  Variables: independent and dependent variables, qualitative and quantitative variables, discrete and continuous variables, and methods of controlling variables. Measurement of variables.
  •  Sampling: Meaning, characteristics of good sample design, steps in sample design, types and advantages.
  • Techniques of data collection:Primary data: Questionnaire, schedules, interview and observation; Secondary data

Statistical Analysis

·         Measure of Central Tendency- Mean, Median, Mode & their properties, merits and demerits.

·         Measure of Dispersion- Range, Quartile Deviation, Mean Deviation and Standard Deviation

·         Small sample test- Student’s t-test and Chi-square test

Analysis of Variance- Concept of ANOVA, its assumptions, Basic idea of one-way and two-way classification 

12.00
Unit IV: 
Correlation and Regression

 

·         Correlation Analysis- Definition and concept, Significance and types of correlation, properties of correlation coefficient

·         Measures of studying correlation

o Karl Pearson’s coefficient of correlation, its       assumptions, properties, merits and demerits

o   Spearman’s Rank correlation coefficient •

·         Regression Analysis-Concept, uses, Regression Coefficients, Regression Equations, Fitting of Regression lines

Linear and multiple regression

Instrumentation

Principle and working of the following equipment:

Spectrophotometry:

o   UV-Visible Spectrophotometer

o   InfraRed (IR) Spectrophotometer

o   Fluorescence Spectrophotometer

o   Atomic Absorption Spectrophotometer (AAS)

o   Mass Spectrometry

o   Flame Photometer

Microscopy:

o   Scanning Electron Microscope(SEM)

o   Transmission Electron Microscopy (TEM)

o    Chromatography:

o    Ion exchange chromatography

o    High Performance Liquid Chromatography

Gas Chromatography

Essential Readings: 

Books Recommended:

·         Plant Biotechnology, Hammond, P. McGarvey and V. Yusibov (Editors.) Springer Verlag, 2000.

·         Plant Cell and Tissue Culture for the Production of Food ingredients. J. Fu, G. Singh, and W.R. Curtis (Editors), Kluwer Academic/Plenum Press.1999.

·         Advanced Methods in Plant Biotechnology, David. R. Murray: Panima Publishing Corporation. 1996

·         Plant Tissue Culture – Techniques & Experiments, (2nd edition) Roberta. H. Smith.  Elsevier. 2000

·         Plant Molecular Biology – A Practical Approach. C. H. Shaw, IRL Press Ltd.

·         Molecular plant Biology- volume 1 & 2. Philip M. Gilmartin and Chris Bowler,  Oxford University Press. 2005

·         Plant Tissue Culture. S.S. Bhojwani and M.K Razdan,  Elsevier. 2004

·         Biotechnology in Crop improvement, H.S. Chawla, International Book Distributing Company, 1998.

·         Practical Application of plant Molecular Biology, R.J. Henry, Chapman

 

 

 

Academic Year: