Practical: Microbiological Analysis & Disaster Management

Paper Code: 
25ENV 325
Credits: 
6
Contact Hours: 
12.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to -

  1. Integrate theoretical and practical knowledge of isolation of microbes from different habitats
  2. Make the students aware of natural disasters and their management

 

Course Outcomes: 

Upon     completion     of     the

practical     course,     student

will:

CO92: Develop understanding regarding working principles and procedures for instruments used in microbiology and biotechnology exercises

CO93: Demonstrate techniques involved           in the isolation of microorganisms from different habitats

CO94: Appraise the natural and anthropogenic disasters and analyze their management process through report preparation and presentation.

CO95: Examine the disasters of the past and critically analyze them through case studies and map plotting

CO96: Accurately document experimental procedures and results in the practical file, ensuring comprehensive and precise records of laboratory activities.

CO97: Explain experimental concepts during viva-voce assessments, demonstrating the practical application of theoretical knowledge.

CO98: Contribute effectively to course-specific interaction

MICROBIOLOGICAL ANALYSIS

  • Acquaintance with the following for spot identification:
    • Autoclave, Incubator, Hot plate, Water bath, Stirrer, Inoculating loop, Oven, Centrifuge, Bunsen burner, Laminar flow hood, Microscope
  • Determination of coliforms and faecal streptococci in water and wastewater by Multiple fermentation techniques
  • Detection of coliforms and faecal coliforms in water by the membrane filtration method
  • Differentiation of members of the family Enterobacteriaceae using IMViC tests
  • Heterotrophic plate count
  • Isolation and enumeration of microorganisms from the soil by serial dilution agar plating method

BIOTECHNOLOGY

  • Thin layer chromatography
  • Gel Electrophoresis

   EXERCISE ON DISASTER MANAGEMENT

  • The students will prepare a report and a PowerPoint presentation on specific disaster management topics of interest (eg. Earthquake, Floods, Drought, Landslide, epidemics, pandemics, anthropogenic disasters, etc.) followed by peer feedback and discussions on presentation content and delivery.
  • Research and compilation of a list of essential items needed in an emergency preparedness kit followed by discussions and review of the contents of the preparedness kit
  • Case study on any recent disaster including  analysis of  the disaster response and recovery efforts, including the successes and failures
  • Earthquake drill: Simulate an earthquake scenario and practice the appropriate safety procedures

 

MAP FILE FOR DISASTER MANAGEMENT

  • Flood-prone areas in India
  • Major floods in India
  • Landslide-prone areas in India
  • Major landslides in India
  • Cyclone-prone areas in India
  • Major cyclones in India
  • Drought-prone areas in India
  • Seismic zones of India
  • Major earthquakes in India
  • Major anthropogenic disasters in India & World
  • Major riots in India
  • Major terrorist attacks in India
  • Major mining accidents in India
  • Major nuclear disasters in the world
  • Major active volcanoes in the world

 

Essential Readings: 

LABORATORY READINGS:

·         Adams, Danny Spencer, LabMath: A Handbook of Measurements, Calculations and Other Quantitative Skills for Use at the Bench

·         Laboratory Manual in General Microbiology-Kannan, N
Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above

 

Academic Year: 
2025-2026
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