Coming soon
Practical
This course will enable the students to –
- Acquaint with working principle and process of instruments used in Environmental Microbiology and Biotechnology.
- Integrate theoretical and practical knowledge of isolation of microbes from different habitats
Course Outcomes (COs):
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Course |
Course outcome |
Learning and teaching strategies |
Assessment Strategies |
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|---|---|---|---|---|
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Paper Code |
Paper Title |
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ENV 303 |
Practical |
Upon completion of this practical course, students will: CO33: Illustrate working principle and procedures for instruments used in microbiology and biotechnology exercises CO34: Explain techniques involved in isolation of microorganisms from different habitats CO35: Develop the practical skills for conducting experiments to assess the microbial load in samples collected from various habitats CO36: Develop a very good understanding of quantification of microbes from various water sources |
Approach in teaching: Conduction of practical exercises, Preparation of sample dilutions, demonstration Learning activities for the students: Simulation, Presentation, Self-learning videos |
Preparation of practical records, Viva Voce, Class Test, Semester End Examination |
CONTENTS
- Preparation of Nutrient Agar and Nutrient Broth
- Isolation of pure culture using Streak Plate Technique
- Isolation of microorganisms from soil sample.
- Isolation of microorganisms from water sample
- Examination of sanitary quality of milk by coliform analysis and methylene blue reductase test
- To study the gram staining of bacteria culture
- Thin layer chromatography
- Paper chromatography
- MPN (Most Probable Number)-for polluted and non-polluted water samples
- Acquaintance with the following for spot identification
- Autoclave
- Laminar flow hood
- Incubator
- Hot plate
- pH meter
- Centrifuge
- Magnetic Stirrer
- Inoculating loop
- Oven
- Bunsen burner
- Qubec Colony counter
Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above.
