PRACTICAL

Paper Code: 
ENV 303
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
  • Preparation of Nutrient Agar and Nutrient Broth
  • Isolation of pure culture using Streak Plate Technique
  • Isolation of microorganisms from soil sample.
  • Isolation of microorganisms from water sample
  • Isolation of microorganisms from food sample
  • Examination of sanitary quality of milk by coliform analysis and methylene blue reductase test
  • To study the gram staining of bacteria culture
  • Thin layer chromatography
  • Paper chromatography
  • MPN (Most Probable Number)-for polluted and non polluted water samples
  • Acquaintance with the following for spot identification
  • Autoclave
  • Incubator
  • Hot plate
  • Water bath
  • Stirrer
  • Inoculating loop
  • Oven
  • Centrifuge
  • Bunsen burner Laminar flow hood
  • Qubec Colony counter

 

Academic Year: