Practical

·         Preparation of Nutrient Agar and Nutrient Broth

·         Isolation of pure culture using Streak Plate Technique

·         Isolation of microorganisms from soil sample.

·         Isolation of microorganisms from water sample

·         Isolation of microorganisms from food sample

·         Examination of sanitary quality of milk by coliform analysis and methylene blue reductase test

·         To study the gram staining of bacteria culture

·         Thin layer chromatography

·         Paper chromatography

·         MPN (Most Probable Number)-for polluted and non polluted water samples

·         Acquaintance with the following for spot identification

o   Autoclave

o   Incubator

o   Hot plate

o   Water bath

o   Stirrer

o   Inoculating loop

o   Oven

o   Centrifuge

o   Bunsen burner Laminar flow hood

o   Qubec Colony counter

Note:  Any other practical exercise (related to theory) may be conducted besides the ones mentioned above.