PRACTICAL

Paper Code: 
ENV 325
Credits: 
08
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 

Course objectives

This course will enable the students to -

  1. Integrate theoretical and practical knowledge of isolation of microbes from different habitats
  2. Become aware about natural disasters and their management

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ENV 325

Practical

Upon completion of the practical course, student will be able to:

CO65:  Develop understanding regarding working principle and procedures for instruments used in microbiology and biotechnology exercises

CO66:  Demonstrate techniques involved in isolation of microorganisms from different habitats

CO67:  Develop a very good understanding of quantification of microbes from various water sources

CO68:  Identify natural disasters and describe their management process

Approach in teaching:

Demonstration, Group discussions, Conducting experiments, field trips

Learning activities for the students:

Brainstorming, preparation of maps, reports, individual and group projects, surveys

Spotting, Quiz

Power Point Presentation, Map Activity, Viva Voce, Class test and Semester end examination

 

 

MICROBIOLOGICAL ANALYSIS

  • Multiple fermentation techqunique- MPN index for polluted and non polluted waters
  • IMVIC tests
  • Heterotrophic plate count
  • Isolation of microorganisms from soil sample

 BIOTECHNOLOGY

  • Acquaintance with the following for spot identification
  • Autoclave, Incubator, Hot plate, Water bath, Stirrer, Inoculating loop, Oven, Centrifuge, Bunsen burner, Laminar flow hood, Tripod with Asbestos mat
  • TLC
  • Gel Electrophoresis

EXERCISE ON DISASTER MANAGEMENT

Students shall be divided into groups

 1.Meteorological :Hailstorm, Snow storm, Dust storm, Wind storm, Fog, Lightening, Heavy rain;

 2.Geological : Earthquake, Landslide, Mudflow, Tsunami, Volcano;

3.Hydrological :Drought ,Flood;

4. Biological:Epidemics, Pest ;

5. Infrastructure :Transport -Aviation ,Railway, Road, Shipping, Energy -Mining ,Nuclear;

6.Industrial :Chemical ,Gas Leak

7. Others :Fire, Riot, Terrorism, War;

Based on the topic within the group students will be guided in their reading on the available literature on theory and practice about these disasters and their management. Students will make group presentation (ppt and a write up) on that topic.

 

 

Essential Readings: 
  • Adams,Dany Spencer,Lab Math:A Handbook of Measurements, Calculations and Other Quantitative Skills for Use at the Bench
  • Laboratory Manual in General Microbiology Kannan, N

 

Academic Year: