Practical

Paper Code: 
DENV 502 A
Credits: 
02
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

This course will enable the students to –

  1. Acquaint with working principle and process of instruments used in Environmental Microbiology and Biotechnology
  2. Integrate theoretical and practical knowledge of isolation of microbes from different habitats

Course Outcomes (COs):

Course

Course outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

DENV 502 A

Practical

Upon   completion             of             this

practical   course,                  students

will:

CO37:       Illustrate                working principle    and                procedure for

instruments used in microbiology

and biotechnology exercises

CO38:      Explain                techniques involved   in the isolation of microorganisms from different habitats

CO39:   Develop   the             practical skills for conducting experiments to assess the microbial load in samples collected from various habitats

CO40: Develop a very good understanding of quantification of microbes from various water

sources

Approach  in teaching: Conduction                     of practical exercises, Preparation of sample dilutions, demonstration

Learning activities for the students: Simulation, Presentation, and Self-learning videos

Preparation of practical records, Viva Voce, Class Test, Semester End Examination

CONTENTS

  • Preparation of Nutrient Agar and Nutrient Broth
  • Isolation of pure culture using Streak Plate Technique
  • Isolation of microorganisms from soil sample.
  • Isolation of microorganisms from water sample
  • Examination of sanitary quality of milk by coliform analysis and methylene blue reductase test
  • To study the gram staining of bacteria culture
  • MPN (Most Probable Number)-for polluted and non-polluted water samples
  • Acquaintance with the following for spot identification
    • Autoclave
    • Laminar flow hood
    • Incubator
    • Hot plate
    • pH meter
    • Centrifuge
    • Magnetic Stirrer
    • Inoculating loop
    • Oven
    • Bunsen burner
    • Qubec Colony counter

Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above

 

Academic Year: 
2023-2024
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