ENVIRONMENTAL MICROBIOLOGY AND BIOTECHNOLOGY

Paper Code: 
ENV 321
Credits: 
5
Contact Hours: 
75.00
Max. Marks: 
100.00
17.00
Unit I: 
Introduction to Environmental Microbiology
  • Definition, scope and application of Environmental Microbiology
  •  Growth of Bacteria in Environment (Lag phase ,Exponential phase and Stationary and Death phase )
  • Types of microorganisms on the basis of Nutrition
  • Microorganisms found in extreme conditions–Extremophiles (acidophiles,psychrophiles , thermophiles and halophiles)
16.00
Unit II: 
Industrial Microbiology
  • Microbial growth kinetics: batch, fed batch and continuous process 
  • Bioengineering of microorganisms for industrial purposes
  •  Industrial use of the following
    • Bacteria (Lactic acid production, Vinegar production, Amino acid production),
  • Yeast (Alcohol fermentation, Bakers yeast and food yeast),
  • Moulds (Penicillin production, Citric acid production, Enzyme production).

 

15.00
Unit III: 
Microbial Transformation of Heavy Metals
  • Heavy metal toxicity in the environment
  • Metal microbes interactions:
  • Intracellular accumulation
  • Cell wall associated metal interaction
  • Metal- siderophore interactions
  • Extracellular mobilization/immobilization of metals by bacterial     metabolites
  • Extracellular polymer-metal interactions
  • Transformation and volatilization of metals

 

12.00
Unit IV: 
Environmental Biotechnology
  • Concept of Recombinant technology
    • Molecular tools of genetic engineering
    • Host cell-Prokaryotic and Eukaryotic
    • Vectors-Plasmids(pBR322),Bacteriophages(λ)Cosmids, BAC amd YAC
    • Methods of gene transfer – Conjugation,Transformation,Transduction
  • Bioremediation-insitu and exsitu

 

15.00
Unit V: 
Biotechnology and Resource Conservation
  • Production of biogas- Biomethanation (Anaerobic treatment for biogas generation, Microbiology and biochemistry, Factors affecting biogas production, Design of digesters);
  • Composting (Types, material, process, physicochemical characteristics of environment, decomposition stages and methods).
  • Vermicomposting; Bioleaching (methods and microorganisms used)
  • Integrated Pest Management (Pest control, Concept of IPM, Biopesticides in IPM).
  • Biosensors-Types and applications

 

Essential Readings: 
  • ·         Atlas, R.M., R. B. (2005). Microbial Ecology: Fundamental and Applications (4th ed.). New Delhi: Pearson Education Pvt. Ltd.
  • ·         Barton, L., D. E. (2011). Microbial Ecology. New Jersey: John Wiley & Sons, Inc., Publication.
  • ·         Chatterji, A. (2011). Introduction to Environmnetal Biotechnology (3rd ed.). New Delhi: PHI Learning Pvt. Ltd.
  • ·         Evans, G. M., & Furlong, J. C. (2003). Environmental Biotechnology: Theory and Application. John Wiley & Sons Ltd.
  • ·         Jjemba, P. K. (2004). Environmental microbiology: principles and applications. Science Publishers.
  • ·         Maier, R.M., I. L. (2009). Environmental Microbiology (2nd ed.). USA: Academic Press (Elsevier).
  • ·         Michael J. Pelczar, E. C. (1993). Microbiology (5th ed.). New Delhi: Tata McGraw-Hill Publishing Company Limited.
  • ·         Mohapatra, P. K. (2008). Textbook of Environmental Environmental Microbiology. New Delhi: I. K. International Publishing House.
  • ·         Mohapatra, P. K. (2010). Textbook of Environmental Biotechnology. New Delhi: I.K. International Pvt. Ltd.
  • ·         Prescott, L. M. (2002). Microbiology (5th ed.). The McGraw−Hill Companies.
  • ·         Stanier, R. (1987). General Microbiology (5th ed.). Palgrave Macmillan .
  • ·         Wainwright, M. (1999). An Introduction To Environmental Biotechnology. New York: Springer Science+Business Media, Llc.

 

Academic Year: 
2017-2018
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