Practical

Practical [1]

Paper Code:

ENV 303

Credits:

Contact Hours:

60.00

Max. Marks:

100.00

Objective:

Course objectives:

This course will enable the students to –

1. Acquaint with working principle and process of instruments used in Environmental Microbiology and Biotechnology

2. Integrate theoretical and practical knowledge of isolation of microbes from different habitats

Course Outcomes (COs):

Course	Course outcome	Learning and teaching strategies	Assessment Strategies
Paper Code	Paper Title
ENV 303	Practical	Upon completion of this practical course, students will: CO33: Illustrate working principle and procedures for instruments used in microbiology and biotechnology exercises CO34: Explain techniques involved in isolation of microorganisms from different habitats CO35: Develop the practical skills for conducting experiments to assess the microbial load in samples collected from various habitats CO36: Develop a very good understanding of quantification of microbes from various water sources	Approach in teaching: Conduction of practical exercises, Preparation of sample dilutions, demonstration Learning activities for the students: Simulation, Presentation, Self learning videos	Preparation of practical records, Viva Voce, Class Test, Semester End Examination

Course

Course outcome

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ENV 303

Practical

Upon completion of this practical course, students will:

CO33: Illustrate working principle and procedures for instruments used in microbiology and biotechnology exercises

CO34: Explain techniques involved in isolation of microorganisms from different habitats

CO35: Develop the practical skills for conducting experiments to assess the microbial load in samples collected from various habitats

CO36: Develop a very good understanding of quantification of microbes from various water sources

Approach in teaching:

Conduction of practical exercises, Preparation of sample dilutions, demonstration

Learning activities for the students:

Simulation, Presentation, Self learning videos

Preparation of practical records, Viva Voce, Class Test, Semester End Examination

CONTENTS

Preparation of Nutrient Agar and Nutrient Broth
Isolation of pure culture using Streak Plate Technique
Isolation of microorganisms from soil sample.
Isolation of microorganisms from water sample
Examination of sanitary quality of milk by coliform analysis and methylene blue reductase test
To study the gram staining of bacteria culture
Thin layer chromatography
Paper chromatography
MPN (Most Probable Number)-for polluted and non polluted water samples

Note: Any other practical exercise (related to theory) may be conducted besides the ones mentioned above.

Academic Year:

Follow Environmental Science on:

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India Phone:- +91-141-2400160-61, 2397906-07, Fax: 2395494, 2781158